Multi-Channel Patterning of Antibodies on a Sensor Surface for Simultaneous Detection of Pathogens and Toxins

Davies, M. J., McDonnell, M.B., Johnston, I.D., Perkins, E.A. and Tracey, M.C. (2010) Multi-Channel Patterning of Antibodies on a Sensor Surface for Simultaneous Detection of Pathogens and Toxins. UNSPECIFIED.
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This poster describes the development of a micro-engineered, PDMS/PMMA composite patterning device for the flow immobilisation of up to 16 separate antibody stripes on a single sensor chip surface. Results for a preliminary six channel version of the device will be described with antibodies immobilised on a commercially available BIAcore (TM) surface plasmon resonance chip. Testing was carried out using a range of bacteria, viruses and proteins using a Light-Scattering SPR instrument. The ultimate aim is to use the device to pattern multiplex sensor chips with antibodies for application in the rapid detection of a range of pathogenic microorganisms and toxins in the field.