Early stages in cell wall regeneration of tobacco mesophyll protoplasts : An electrophoretic study
The electrophoretic mobilities of isolated mesophyll protoplasts of Nicotiana tabacum var. Xanthi n.c. were measured using a whole cell electrophoresis apparatus. Changes in mobility were recorded at times during aseptic culture which related to events in the known sequence of de novo cell wall regeneration. It is proposed that these changes are a result of activity in, or at, the plasmalemma resulting in synthesis and transport to the cell surface of regenerated cell wall material. Coumarin, a compound known to affect the developmental sequence of cultured mesophyll protoplasts, was examined as a medium component during the regenerative process, differences in protoplast mobility resulting from Coumarin addition were recorded. The possible mode of action of Coumarin is discussed
Item Type | Article |
---|---|
Date Deposited | 26 Jul 2024 17:03 |
Last Modified | 26 Jul 2024 17:03 |
Explore Further
Read more research from the creator(s):
Find work associated with the faculties and division(s):
- Geography, Environment and Agriculture
- Agriculture, Food and Veterinary Sciences
- Crop Protection and Climate Change
- School of Life and Medical Sciences
- Health & Human Sciences Research Institute
- Department of Human and Environmental Sciences
Find other related resources: