Electrowetting-Based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
Electrowetting is the effect by which the contact angle of a droplet exposed to a surface charge is modified. Electrowetting-on-dielectric (EWOD) exploits the dielectric properties of thin insulator films to enhance the charge density and hence boost the electrowetting effect. The presence of charges results in an electrically induced spreading of the droplet which permits purposeful manipulation across a hydrophobic surface. Here, we demonstrate EWOD-based protocol for sample processing and detection of four categories of antigens, using an automated surface actuation platform, via two variations of an Enzyme-Linked Immunosorbent Assay (ELISA) methods. The ELISA is performed on magnetic beads with immobilized primary antibodies which can be selected to target a specific antigen. An antibody conjugated to HRP binds to the antigen and is mixed with H 2O 2/Luminol for quantification of the captured pathogens. Assay completion times of between 6 and 10 min were achieved, whilst minuscule volumes of reagents were utilized.
Item Type | Article |
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Uncontrolled Keywords | Automation; Biodetection; Chemiluminescence; DMF; Digital microfluidics; ELISA; EWOD; Electrowetting on Dielectric; Enzyme-Linked Immunosorbent Assay; Issue 156; Pathogen quantification |
Subjects |
Chemical Engineering(all) > Bioengineering Neuroscience(all) Chemical Engineering(all) Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) |
Date Deposited | 26 Jul 2024 21:18 |
Last Modified | 26 Jul 2024 21:18 |
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Find work associated with the faculties and division(s):
- Centre for Engineering Research
- Centre for Research in Biodetection Technologies
- Micro Electro-Mechanical Systems
- Microfluidics and Microengineering
- BioEngineering
- School of Physics, Engineering & Computer Science
- Department of Engineering and Technology
- School of Engineering and Technology
- SPECS Deans Group
- Centre for Hazard Detection and Protection Research
- Extracellular Vesicle Research Unit
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